"Further to the injury sustained during our match at Manchester United on Wednesday night, we can confirm that Rob Holding has ruptured the anterior cruciate ligament of his left knee," Arsenal said in a statement.
"Rob will undergo surgery to repair this in the coming days. The rehabilitation process is expected to take between six to nine months.
"Everyone at the club will now be working as hard as we can to ensure Rob is back on the pitch as soon as possible."
Holding's injury could speed up club captain Laurent Koscielny's return to the starting team, with the Frenchman fit again after undergoing surgery on his Achilles tendon in May.
Arsenal are fifth in the table with 31 points from 15 matches and host Huddersfield Town in the league on Saturday.
(Reporting by Shrivathsa Sridhar in Bengaluru; Editing by Toby Davis)
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