Go-Jo, whose real name is Marty Zambotto, opened the second semi-final with his song Milkshake Man on Friday morning (AEST).
Viewers chose their favourite performances via televote, and 10 countries went through out of the 16 in the second semi-final.
Lithuania, Israel, Armenia, Denmark, Austria, Luxembourg, Finland, Latvia, Malta and Greece were the 10 countries that made it through.
In addition to Australia, Montenegro, Ireland, Georgia, Czechia and Serbia were also knocked out of the contest in the second semi-final.
Go-Jo spoke to SBS News ahead of the semi-final, and was asked what success would look like for him in this year's contest.
"I think just seeing smiles," he replied, adding that it would be a reflection of the hard work that went into the performance.
"A lot of people would see me, as the performer, but I have an incredible team behind everything.
I think one of the most meaningful things is making them proud, and obviously Australia. It's such a big thing for me. Just, seeing everyone smile at the end of everything, I think that's a big win."
It's the second year in a row Australia has been knocked out of the contest during the semi-final, with 2024 entrants Electric Fields also not qualifying.
The last time Australia made it through to the grand final was in 2023, with progressive metal band Voyager and their song Promise. It finished in ninth place, with Sweden taking out the top spot.
When will the Eurovision 2025 grand final be?
You can watch the Eurovision 2025 grand final on Sunday 18 May at 5am AEST on SBS and SBS On Demand.
Who will compete in the Eurovision 2025 grand final?
In addition to the 10 qualifiers listed above, Sunday's grand final will also feature the 10 qualifiers from the first semi-final: Iceland, Poland, Estonia, Ukraine, Sweden, Portugal, Norway, San Marino, Albania, and the Netherlands.
Host country Switzerland, as well as the 'big five' of France, Germany, Italy, Spain and the United Kingdom, all of whom automatically qualified, will also compete.
You can listen to every Eurovision winner and this year's entries on SBS EuroPop — our 24/7 Eurovision music station, running for the entire month of May.
