Australians Samantha Stosur, Daria Gavrilova and Anastasia Rodionova have all been knocked out of the Charleston Open in South Carolina.
Sixth-seed Stosur hit 24 unforced errors in her 7-5 6-3 loss to Romanian world No.33 Irina-Camelia Begu in the round-of-16 clash on green clay on Thursday (Friday AEST).
Begu sent down five aces to Stosur's one in the opening set before breaking the 2011 US Open winner twice in the second to coast to a straight-set victory.
World No.27 Gavrilova went down to Russian Daria Kasatkina 6-3 4-6 6-0 in a match that lasted more than two hours.
Both players struggled to find their rhythm on serve in a see-sawing opening two sets.
The unseeded Kasatkina saved all four of Gavrilova's break points in the decider as the Australian continued her serving woes.
Kasatkina, who will meet Begu in the quarter-finals, won 14 of 19 break points overall, while Gavrilova converted just two of 10.
"It was very difficult. I was playing against my very good friend, so it was like double portion of toughness," Kasatkina said.
"The main key was, I think, the break points. I won almost every break point."
Qualifier Anastasia Rodionova was also beaten to end the Australian charge, going down to fifth seed and former world No.1 Caroline Wozniacki 6-3 6-3.
In other results, Latvia's eighth Anastasija Sevastova did it the hard way against Tunisia's Ons Jabeur 7-5 7-6 (8-6), Mirjana Lucic-Baroni of Croatia beat Dutch seventh seed Kiki Bertens 7-6 (7-5) 6-4 and German Laura Siegemund upset Lucie Safarova of the Czech Republic 6-2 6-3.
Fanny Stollar was forced to retire from her match against Jelena Ostapenko.
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