The 17 local hopes know their Australian Open first-round matchups and venues after the end of qualifying on Sunday.
No Australians made it through qualifying, with Bernard Tomic coming closest before falling in three sets to Italian Lorenzo Sonego in his third and final match.
Nick Kyrgios starts his campaign on Hisense Arena against Rogerio Dutra Silva.
Australia's top females, Ashleigh Barty and Daria Gavrilova, will enjoy playing on Rod Laver Arena over the first two nights of the tournament.
FIRST-ROUND DRAW FOR AUSTRALIANS AT MELBOURNE PARK
DAY ONE: MONDAY 15 JANUARY
* 17-Nick Kyrgios v Rogerio Dutra Silva (BRA) - Hisense Arena
* Matthew Ebden v 16-John Isner (USA) - Margaret Court Arena
* John Millman v Borna Coric (CRO) - show court 3
* Alex Bolt v Viktor Troicki (SRB) - court 7
* Jason Kubler v 10-Pablo Carreno Busta (ESP) - court 8
* 23-Daria Gavrilova v Irina Falconi (USA) - Rod Laver Arena
* Samantha Stosur v Monica Puig (PUR) - Margaret Court Arena
* Jaimee Fourlis v Olivia Rogowska - show court 3
DAY TWO: TUESDAY 16 JANUARY
* Alex de Minaur v Tomas Berdych (CZE) - Hisense Arena
* Thanasi Kokkinakis v Daniil Medvedev (RUS) - show court 3
* Jordan Thompson v Nicolas Kicker (ARG) - court 8
* Alexei Popyrin v Tim Smyczek (USA) - court 14
* Destanee Aiava v 1-Simona Halep (ROM) - Rod Laver Arena
* 18-Ashleigh Barty v Aryna Sabalenka (BLR) - Rod Laver Arena
* Ajla Tomljanovic v 29-Lucie Safarova (CZE) - show court 3
* Lizette Cabrera v Beatriz Haddad Maia (BRA) - court 7
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