The 26-year-old found himself called into action earlier than expected, with the Scorchers struggling on 16 for three in the fourth over against the Hobart Hurricanes.
Australian media reported that parts of the Tasmanian crowd erupted in a chorus of boos when Bancroft was announced as the incoming batsman.
Facing fast bowler Riley Meredith, Bancroft clipped the first ball off his pads for two runs. He blocked the second delivery, before edging the third to wicketkeeper Matthew Wade as the Scorchers fell to 19-4.
Bancroft will have a chance to earn an Australian test recall when he resumes as a top-order batsman for Western Australia in the Sheffield Shield in February.
(Reporting by Hardik Vyas in Bengaluru; Editing by John O'Brien)
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