Midfielder Iniesta had to be replaced by Xavi eight minutes into the second half of Wednesday's Champions League quarter-final first leg at Paris St Germain, after tangling with PSG midfielder Javier Pastore.
"He is much better but he didn’t train so in principle and barring a miracle he won’t be in the squad," Luis Enrique told a news conference.
Barca can go five points clear of second-placed Real Madrid, who host Malaga in a later kickoff, with a win at the Nou Camp, although it promises to be a tough match as Valencia are fourth and on a 10 match unbeaten run.
In his first season in charge, Luis Enrique's side have a chance of repeating 2009's treble of Champions League and Spanish league and Cup titles.
(Writing by Iain Rogers in Madrid, editing by Douglas Beattie)
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