The FA said in a statement Bertrand was "returning to Southampton for further assessment on a back issue.
"Bertrand's withdrawal is a precautionary measure, but he won't play in this month's matches."
The left-back's withdrawal leaves Danny Rose and Ashley Young competing for a starting spot as players look to show they are worth a place in England's squad for the World Cup.
(Reporting by Aditi Prakash in Bengaluru; Editing by Peter Rutherford)
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