The Finn, who qualified sixth at Albert Park, felt lower back pain during the session and was undergoing precautionary checks at Melbourne's Alfred Hospital, his Formula One team said.
A spokeswoman said his condition would be assessed at the track before Sunday's race.
"Valtteri was suffering with his back and lost a lot of time down at the last corner, coming out," team performance chief Rob Smedley said.
"We don’t know what it is. He just started complaining about it in the middle of the second qualifying, saying the small of his back was hurting.
"We had a look behind his seat and there was nothing in there. I don’t know what’s happened,, whether he’s jarred his back or something. So that’s not going very well."
Bottas' Brazilian team mate Felipe Massa qualified third behind world champion pole-sitter Lewis Hamilton and Mercedes team mate Nico Rosberg.
(Editing by Alan Baldwin)
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