"Pascal Dupraz and (club president) Olivier Sadran have decided to end their collaboration this morning," Toulouse said in a statement.
"Mickael Debeve will take the reins of the professional team."
Toulouse are second bottom of the Ligue 1 standings after a 2-1 defeat at Montpellier on Saturday, two points from safety.
Debeve played for Toulouse from 1986-94 and was Dupraz's assistant coach.
(Reporting by Julien Pretot,; Editing by Ed Osmond)
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