"Rojo has a hamstring injury and Paddy McNair has a minor injury," the manager told the club's website (www.manutd.com).
The injuries to two more defenders mean that Phil Jones could make his first appearance of the season for United, despite not being fully fit.
The 23-year-old has been sidelined with mild thrombosis -- a blood clot -- but has been included in the squad and will be on the bench for the tie against the Championship side.
"I have to admit that because of the injury of three defenders (including Luke Shaw), I shall have Jones on the bench but he is not fit," Van Gaal said.
(Reporting by Simon Jennings in Bengaluru; editing by Sudipto Ganguly)
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