The French winger was taken off in the 29th minute for Gerard Deulofeu, with the scoreline goalless at the Coliseum Alfonso Perez.
"Ousmane Dembele has a hamstring injury in his left leg, more tests are needed to find out the extent of the injury," said Barcelona in a statement.
Dembele, Barcelona’s record signing, had started against Juventus in the club’s 3-0 Champions League win on Tuesday.
Reports in Spanish media suggested the 20-year-old would miss three weeks, which includes league fixtures against Eibar, Girona and Las Palmas, as well as a Champions League clash in Lisbon against Sporting.
Dembele became Barcelona’s record signing in August when the Catalans paid Borussia Dortmund a fee which could reach 147 million euros ($175.53 million) with add-ons, after Neymar departed for Paris Saint-Germain.
(Reporting by Rik Sharma; Editing by Clare Fallon)
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