Serbian world number one Djokovic has reached the U.S. Open men's singles final for the past four years at Flushing Meadows, winning the title in 2011.
Swiss Federer is seeded at the U.S. Open for a 14th consecutive year, tying Ivan Lendl for the second longest streak in the Open era with only Jimmy Connors (18) ahead of them.
Spanish world number two Rafa Nadal, the reigning U.S. Open champion, has withdrawn from the tournament because an injured right wrist.
Australian Open champion Stan Wawrinka of Switzerland has been seeded third with Spaniard David Ferrer fourth and big-serving Canadian Milos Raonic fifth.
Czech Tomas Berdych is the sixth seed, Bulgaria's Grigor Dimitrov seventh and Britain's Andy Murray, the 2012 U.S. Open champion, eighth.
The seeds for the women's tournament will be announced on Wednesday, with the draw to be conducted at the USTA Billie Jean King National Tennis Center on Thursday.
The U.S. Open starts on Monday.
(Reporting by Mark Lamport-Stokes in Los Angeles; Editing by Gene Cherry)
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