Kromowidjojo, Olympic champion at 50 and 100 freestyle at last year's London Games, clocked a time of 24.05 seconds.
Cate Campbell of Australia, who won gold in the 100 freestyle on Friday, took silver in 24.14 and Briton Francesca Halsall claimed the bronze in 24.30, her nation's first medal of the championships.
(Reporting by Iain Rogers, editing by Clare Fallon)
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