Coach Reinaldo Rueda picked 24 players including injured first choice goalkeeper Alexander Dominguez for the friendly in Amsterdam on May 17.
Striker Armando Wila has earned a first call-up as top scorer with 11 goals in the Ecuadorean first division playing for Universidad Catolica.
Forward Jaime Ayovi has been recalled and 19-year-olds Cristian Ramirez of Fortuna Duesseldorf, capped twice, and the uncapped Carlos Gruezo of VfB Stuttgart have also been included.
“Each call-up excites players who are in competitive football and have shown they deserved it,” said Colombian Rueda, whose team face France, Switzerland and Honduras in Group E at the finals in Brazil starting on June 12.
Dominguez, first choice in goal during the qualifying series, is recovering from a broken wrist he suffered playing for his club LDU Quito last month.
Ecuador have also lined up warm-ups against Mexico on May 31 and England on June 4.
The Dutch are in Group B with title holders Spain, Chile and Australia.
Squad:
Goalkeepers: Maximo Banguera (Barcelona SC), Alexander Dominguez (LDU Quito), Adrian Bone (El Nacional)
Defenders: Oscar Bagui (Emelec), Gabriel Achilier (Emelec) Frickson Erazo (Flamengo), Walter Ayovi (Pachuca), Jorge Guagua (Emelec), Juan Carlos Paredes (Barcelona SC), Cristian Ramirez (Fortuna Duesseldorf)
Midfielders: Segundo Castillo (Al-Hilal), Carlos Gruezo (VfB Stuttgart), Renato Ibarra (Vitesse Arnhem), Fidel Martinez (Tijuana), Cristian Noboa (Dynamo Moscow), Pedro Quinonez (Emelec), Luis Saritama (Barcelona SC), Antonio Valencia (Manchester United)
Forwards: Felipe Caicedo (Al-Jazira), Jefferson Montero (Morelia), Joao Rojas (Cruz Azul), Armando Wila (Universidad Catolica), Enner Valencia (Pachuca), Jaime Ayovi (Tijuana)
(Reporting by Alexandra Valencia; Writing by Rex Gowar in Buenos Aires, editing by Pritha Sarkar)
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