The FA said in a statement on Sunday that Chambers, who like Stones can operate at centre or right back, had been called up from the Under-21s.
Stones, who was carried off late in the 2-1 defeat by Manchester United, has ankle ligament damage.
Manager Roberto Martinez told reporters the 20-year-old would be out for "at least a few weeks".
(Editing by Rex Gowar/Mark Meadows)
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