The 18-year-old powered home in a time of 58.42 seconds at the Palau Sant Jordi following Sunday's 4x100 metres freestyle relay triumph.
Australia's Emily Seebohm, second behind Franklin at the Olympics in London last year, was second in 59.06 seconds and Aya Terakawa of Japan took bronze in 59.23.
(Reporting by Iain Rogers; Editing by John Mehaffey)
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