The 23-year-old Peaty, who has labelled his ambitions to crack 57 seconds for the race as Project 56, clocked 58.84 seconds to beat fast-finishing compatriot James Wilby to defend the Commonwealth Games title he won in Glasgow four years ago.
Wilby clocked 59.43 seconds with South Africa's Cameron van der Burgh taking bronze 0.01 seconds behind the Englishman.
Peaty had swum a Commonwealth Games record 58.59 on Friday but appeared to be content to just win the race rather than challenge his own world record of 57.13 that he set at the Rio Olympics in 2016.
(Reporting by Greg Stutchbury; Editing by Sudipto Ganguly)
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