"I bet France is really proud of these guys right now," Williams said on behalf of the Gallic pair, who took to the stage with their trademark helmets.
"I suppose the robots would like to say thanks," he said. The pair made no comment from behind their shiny black visors.
They beat rivals rockers Imagine Dragons with "Radioactive," Kiwi singer Lorde with "Royals," Hawaiian pop star Bruno Mars with "Locked Out Of Heaven" and Robin Thicke featuring T.I. and Williams again with "Blurred Lines."
Earlier the French pair, in their first TV appearance in six years, had turned the 56th annual Grammys into a giant disco, performing their mega-hit with Williams and veteran star Stevie Wonder.
Music's A-listers in the first few rows of the audience danced along as the duo appeared inside a mocked up recording booth, backing up Wonder and Williams.
Former Beatles Paul McCartney and Ringo Starr could be seen swaying, as were hip hop's first couple Jay-Z and Beyonce, Katy Perry and many others.
The last TV appearance by Daft Punk -- real names Guy-Manuel de Homem-Christo and Thomas Bangalter -- was in 2008, also at the Grammys.
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