"It's one of the toughest games we could have and in any way that we are going to win it we've got to put in a performance that will give us a chance," Hughton told local daily The Argus.
"So if anything I will look to put out the strongest side that I can to get a result... I have decisions to make, but I'm also very conscious it's Manchester United at Old Trafford."
Midfielder Dale Stephens missed the 2-0 league defeat by Everton over the weekend with a minor knock in training but Hughton expects him to recover in time for Saturday's game.
Brighton come into the clash with Jose Mourinho's men after defeating Coventry City 3-1 in the fifth round while United defeated Huddersfield Town 2-0.
(Reporting by Shrivathsa Sridhar in Bengaluru; editing in Sudipto Ganguly)
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