When asked if Aguero's season with City was over, Guardiola said: "Here, yeah. It's four or five weeks (out). We will try and get him ready for the World Cup.
"He is in Barcelona right now, for the first part of the rehabilitation he is going to be there."
Aguero is expected to be a key figure for Argentina, who play their opening World Cup match against Iceland on June 16.
City were crowned Premier League champions last weekend and Guardiola wants his team to break records before the end of the campaign.
They could finish with the most points, most wins and most goals in a single Premier League season and the Spanish manager remains keen to keep his players motivated ahead of Sunday's clash with Swansea City.
"In the past when I won the league early at Bayern Munich and Barcelona, the next few games were not good," Guardiola added.
"But a good argument to keep going is the records. It would be nice to finish good in terms of good feelings.
"The important thing is to win the title but maybe the records can help us be focussed against the teams fighting to avoid relegation."
City's opponents Swansea are currently 17th in the league and four points clear of the relegation zone with five matches to play.
(Reporting by Hardik Vyas in Bengaluru; Editing by Toby Davis)
