Liverpool manager Juergen Klopp said Henderson had "no chance" of playing for his country in Germany on March 22 or in the World Cup qualifier against Lithuania at Wembley Stadium four days later.
"The (international) break, these two weeks, hopefully – it’s what we hope - (we) can bring him back, but there's no game possible," Klopp told reporters on Sunday after Liverpool's 2-1 home win over Burnley.
"He cannot train, he for sure cannot play against City and it makes sense that he is doing the rehab. Hopefully, he can run in the second week of the international break and then we have to see."
Southgate is scheduled to name his squad on Thursday.
Henderson, who has 32 caps, has played every minute of Southgate's four games in charge so far.
(Writing by Ken Ferris; Editing by Nick Mulvenney)
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