First-half efforts from Daniele Rugani, Gonzalo Higuain and Dani Alves and a late own goal from Luca Ceppitelli helped Juve ease to their fourth win of the season and up to 12 points, one ahead of Napoli on 11.
Inter Milan beat Empoli 2-0 away after a first-half double from Mauro Icardi to go fifth on 10 points, while Roma jumped to third, also on 10, after thrashing Crotone 4-0.
Juve were criticised after their loss to Inter on Sunday and boss Massimiliano Allegri made six changes to his team, with record signing Gonzalo Higuain restored to the starting lineup.
However, it was defender Rugani who opened the scoring with a poacher's finish, reacting first to tap past Marco Storari after the keeper saved from Higuain's volley.
Higuain did get on the scoresheet in the 34th minute when he fired home the rebound after Storari had blocked Miralem Pjanic's effort.
Dani Alves added a third six minutes later with his first for Juve, lashing the ball into the bottom corner from the edge of the box after being picked out by a Pjanic corner.
"I'm happy to return to the top of the table and we played well," Allegri told Sky Sport Italia. "The boys took the right approach, although maybe when we were 2-0 up we should've controlled the tempo instead of pushing forward looking for a third goal too unreservedly."
Juve replaced goalkeeper Gianluigi Buffon at halftime due to injury, but added to their tally in the 83rd minute when Ceppitelli deflected the ball past Storari.
"It was a good response to the rather excessive criticism of our performance against Inter on Sunday," Allegri concluded.
NAPOLI FALL SHORT
Napoli, Serie A's top scorers heading into Wednesday, were held at Genoa despite enjoying the lion's share of possession and were left ruing two penalty appeals that were turned down.
Marek Hamsik struck the woodwork as Maurizio Sarri's side were forced to settle for a point, with a foul on Arkadiusz Milik in the box and Lucas Ocampos's handball going unpunished.
"We commanded the match for 85 minutes," Sarri lamented. "It does disappoint me that I am the one who always has to talk about it, but we should’ve had two penalties."
Inter took the lead against Empoli after 10 minutes when Icardi met Antonio Candreva's delightful chipped cross with a diving header.
They doubled their advantage seven minutes later when Icardi was picked out by Mario and slotted calmly past Lukasz Skorupski for his sixth goal of the season.
"I want to see (this attitude) not just against a great side like Juve, but also other teams," said Inter coach Frank de Boer. "We've proved that we're in good shape."
At the Stadio Olimpico, Roma eased past promoted Crotone in a 4-0 triumph that hinted that Luciano Spalletti's side could be title contenders again.
Stephan El Shaarawy struck in the 26th minute after a sustained period of pressure from the home side, while Mohamed Salah added a second with a thunderous left-footed finish from close range 11 minutes later.
Edin Dzeko bagged two in the second half, the first after a remarkable assist from Francesco Totti, while Wojciech Szczesny saved Raffaele Palladino's penalty.
(Reporting by Ed Dove; Editing by Toby Davis and Pritha Sarkar)
