"Jeannie Longo, who is greatly affected by unfair accusations made against her, is not in a physical or mental condition to defend her chances at the highest level," her lawyers Bruno Ravaz and Pierre Albert wrote in a statement.
"As a consequence, she will not take part in the world championships."
The world championships will be held in Copenhagen from Sept 19-25.
Longo is set to face disciplinary action from the French federation (FFC) once the FFC has received formal notification from the French Anti-Doping Agency (AFLD) that she failed to provide her whereabouts to authorities on three occasions.
In a separate case, her husband Patrice Ciprelli has been provisionally suspended by the FFC after sports daily L'Equipe reported on Tuesday that he bought erythropoietin (EPO) in China via a former U.S. professional rider.
Ciprelli has denied any wrongdoing.
Longo, France's most successful cyclist and one of the nation's favourite sports personalities, won the first of her 13 world road and track titles in 1985 and picked up an Olympic road-race gold medal in Atlanta in 1996.
(Reporting by Julien Pretot; Editing by Alan Baldwin)
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