The 27-year-old notched up 12 goals and 21 assists last term to help Pep Guardiola's side win the league and the League Cup.
"Kevin is a great player and it's just unfortunate that it's happened to him in training but there's more than enough depth in the squad," Walker told reporters.
"We don't just rely on one player. It's a team game and whoever steps in will do well. Obviously, it's a big loss but we've got more than enough cover."
De Bruyne, a World Cup semi-finalist with Belgium earlier this year, is likely to be sidelined for around two to four months, according to British media reports.
City beat Arsenal 2-0 in their opening league fixture last weekend and will host Huddersfield Town on Sunday.
(Reporting by Aditi Prakash in Bengaluru; editing by Sudipto Ganguly)
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