The 16-year-old Olympic champion touched in a time of one minute 04.42 seconds, seven hundredths slower than Monday's blistering effort of 1:04.35.
Yuliya Efimova of Russia took silver in 1:05.02, matching her result from the 2009 championships in Rome, and American Jessica Hardy, who held the world record before Meilutyte, bronze in 1:05.52.
(Reporting by Iain Rogers, editing by Pritha Sarkar)
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