NAB expects 170 to 180 employees to leave the bank if it proceeds with proposed roster changes for customer advisor roles.
The lender on Tuesday confirmed an ABC report it had plans to reshape its workforce to better suit times customers were banking, including changing the hours of customer advisors.
The bank said the changes weren't locked in, with employee consultation underway.
NAB retail general manager Krissie Jones said no-one would leave unless they chose to.
"Some may choose to move into relief roles, to work more or less hours, or move into different roles across NAB," Ms Jones said.
"Some people may choose to leave NAB if the time is right for them."
NAB did not say whether the anticipated job losses were part of the 4,000 planned net reduction in its workforce announced in November 2017.
About 1,900 people left the company in 2018.
The bank's announcement comes just weeks after chairman Ken Henry and chief executive Andrew Thorburn resigned after being dragged over the coals at the banking royal commission.
Shares in NAB were trading 0.83 per cent lower at $24.515 at 1530 AEDT on Tuesday.
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