The top-seeded Osaka was beaten by Czech Siniakova, the world’s top-ranked doubles player, 6-4 6-2 on a hot day at the Court Suzanne-Lenglen at Roland Garros.
Osaka, who has yet to reach the second week in four appearances in Paris, got broken three times and failed to take her chances. Despite entering Roland Garros as world No. 1 for the first time, Osaka was taken to three sets in her first two matches.
She rallied back to beat world No. 90 Anna Karolina Schmiedlova of Slovakia in the first round after losing the first set, and recovered from a set and a break down in a second-round clash against fellow double Grand Slam winner Victoria Azarenka of Belarus.
With back-to-back titles at the US Open and the Australian Open, the 21-year-old from Japan had been trying to do something not even the greatest players in tennis history have done: winning their first three Grand Slam singles title in a row.
The 42nd-ranked Siniakova, the reigning French Open and Wimbledon doubles champion, showed off her doubles credentials in the opening set, unsettling her opponent with sliced forehands and drop shots.
Breaking serve at 4-4, Siniakova clinched the first set in 44 minutes on her second set point as Osaka dumped a forehand into the net. Osaka struggled to settle in the second set, going down a double break with a double fault.
Serving for her first win over a world No. 1 at 5-2, Siniakova kept her nerve and won the match on Osaka's 38th error.
(Reporting by Danielle Rossingh; Editing by Hugh Lawson)
