The Brazilian Football Confederation released a video that showed the Paris St Germain player training with the rest of the squad before they flew off to St Petersburg for Friday's Group E match against Costa Rica.
Brazil drew their opening match 1-1 with Switzerland and Costa Rica lost their opener 1-0 to Serbia, who now top the group.
Neymar became the most expensive player in the world last year when he joined PSG from Barcelona for a fee of 222 million euros (£195 million).
He missed a large part of the season with a fractured right foot and sprained ankle but returned to play in Brazil's final two friendlies before the World Cup began.
(Reporting by Andrew Downie, editing by Pritha Sarkar)
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