Punters will not be able to watch Victorian horse races on television at home, in pubs or clubs after a broadcast deal expired.
But the races can still be seen in the 92 Victorian TAB retail agencies that are not in a pub or club.
Racing Victoria, the state's principal racing authority, is also making vision available through the racing.com website.
Vision is also available on the websites of bookmakers Sportsbet.com.au and CrownBet, via mobile phone, tablets and desktop computers.
Wagering giant Tabcorp says interim broadcasting arrangements between the company and the Victorian racing industry expired at midnight on Monday.
A new broadcast deal with the Victorian thoroughbred industry has not been reached.
"As a result, while wagering on Victorian thoroughbred racing will continue to be available, it will not be supported by vision on (Tabcorp's) Sky Racing channels, outside of the 92 Victorian TAB agencies," Tabcorp said on Tuesday.
"Sky Racing is putting in place measures to minimise the impact on our customers."
More than 600 pubs and clubs with TAB facilities will no longer get vision of Victorian horse races, but audio and printed race results can still be accessed on TV screens.
Vision of races outside Victoria will still be available.
Tabcorp says it does not know yet if the loss of vision of Victorian horse races will affect wagering revenue.
Tabcorp shares dropped eight cents, or 1.7 per cent, to $4.66.
Tabcorp has been in talks on new broadcasting arrangements for Victorian horse races since the beginning of the year.
It submitted a formal offer to the Victorian thoroughbred racing industry on June 1, 2015.
The company said the package included significant monetary benefits, and the opportunity and flexibility to have up to 44 meetings each year broadcast on Channel Seven and other free-to-air television.
Racing Victoria's preferred outcome for the broadcasting of Victorian horse races is a combined free-to-air and digital broadcast proposal from Seven West Media and the maintenance of the Sky Racing broadcast.
Racing Victoria, the Melbourne Racing Club, Moonee Valley Racing Club and Country Racing Victoria have committed to the Seven West proposal.
But the Victorian Racing Club (VRC), which runs racing at Flemington and hosts the Melbourne Cup carnival, has not agreed to terms.
Without the VRC's signature, a proposal to show the racing.com digital service on Seven cannot happen.
VRC chairman Michael Burn said on racing.com on Tuesday that he was supportive of the Seven West proposal, but the VRC was not yet satisfied that what had been put to them represented "fair value" for the VRC.
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