It comes after 30 more countries ratified it at a special session at the United Nations.
The climate deal was struck last year in the French capital, Paris, agreed to by 195 countries.
It lays out a global plan to take steps aimed at limiting climate change.
However it will only come into force when at least 55 nations, representing 55 per cent of global carbon dioxide emissions, ratify it.
Currently only 60 United Nations member states have signed the Paris Agreement.
UN Secretary General Ban Ki-moon says those 60 countries represent more than 47 per cent of the world's greenhouse gas emissions.
"We need 7.5 per cent more. That means we have crossed one of the two thresholds - the 55 countries. So it's well over. Now let us work harder to get the 7.5 per cent greenhouse gas emissions. I thank the 31 countries that have deposited their instrument with me. I'm happy to declare that we have officially crossed one of the two thresholds required to bring the Paris Agreement into force."
The UN says 14 countries, representing 12.58% of emissions, have committed to joining the agreement this year.
If they fulfill that commitment, that would allow the threshold of 55 per cent of global carbon dioxide emissions to be reached.
The binding global deal would cut greenhouse gases, keeping global temperature increases below 2 degrees Celsius.
Scientists warn that countries are likely to cross that threshold if they don't take drastic action.
Each country will reduce their greenhouse gas emissions according to their own national strategies.
The Paris Agreement received a boost earlier this month after the United States and China submitted their plans to join.
The world's two biggest emitters together account for around 40 per cent of global greenhouse gas emissions.
US Secretary of State John Kerry has praised countries that have ratified the agreement.
"Virtually every small island in the Pacific, every island state whose very existence depends on our success, has now joined this agreement. That tells you something. And we are extremely grateful to the 31 others who marched up here and presented."
Fiji was the first country to join the Paris Agreement.
Prime Minister Voreqe Bainimarama says it is crucial that the deal comes into effect soon.
"My country, along with other small island nations, is on the front lines of climate impacts. The rapid entry into force and implementation of the Paris Agreement is critical for our national survival. We have a responsibility to our people and to all the world's people to protect them from rising seas, from powerful storms, from hurricanes and cyclones, from crippling floods, devastating droughts and higher temperatures."
Australia signed the agreement in April, and Prime Minister Malcolm Turnbull is also expressing optimism that the deal is closer to reality.
"We are honouring and fulfilling our commitments made in Paris. I urge other countries to do all they can under their laws to ratify the agreement in 2016 and help bring it into effect. Australia is confident that collectively the world will, in the common good, rise to the challenges ahead."
