"Despite her commitment to the Premier League in early November, Susanna Dinnage has now advised the Nominations Committee that she will not be taking up the position of Chief Executive," read the statement.
"The Committee has reconvened its search and is talking to candidates. There will be no further comment until an appointment is made," concluded the statement.
Executive chairman Scudamore will relinquish his duties at the end of 2018.
The Premier League has decided to split his role, with Dinnage lined up to take over as chief executive before her change of heart.
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Dinnage is currently the global president for Animal Planet at media organisation Discovery Inc. She has previously worked as president for Discovery Networks UK and Ireland.
(Reporting by Simon Evans, editing by Pritha Sarkar)
