Colombian Winner Anacona was victorious in the ninth stage of the Vuelta a Espana on Sunday as his compatriot Nairo Quintana moved ahead in the general classification.
Anacona broke clear on the 8km, first category climb to the finish of the 185km stage from Carboneras de Guadazon to Aramon Valdelinares to move into fourth place overall with Kazakstan's Alexey Lutsenko and Italian Damiano Cunego finishing second and third respectively.
Quintana reacted to an attack from Alberto Contador with a kilometre left to take the overall lead from Movistar teammate Alejandro Valverde.
However, it was a disappointing finish for Chris Froome and Valverde as they lost 23 seconds on the final climb.
Quintana now leads Contador by three seconds with Valverde eight seconds back.
Anacona is just one second further back, but having gained time on his rivals in recent days, Froome dropped to 28sec behind.
"This is a very important moment in my career. It is my first stage victory," said Anacona.
"It is a shame that I couldn't take the overall lead as well, but I just struggled a little on the final stretch but that is a motivation to keep working going forward."
Australia's Adam Hansen finished the stage in eighth place, one minute and 45 seconds behind Anacona, to overtake Cadel Evans as the best-placed Australian in the race at 43rd overall.
Evans, who now trails leader Quintana by 22 minutes and 37 seconds, is in 47th place.
The conditions changed drastically in the first full day away from the searing heat of the southern region of Andalusia as torrential rain fell throughout the stage.
Anacona was one of 31 riders to make an early breakaway from the peloton and he surged away from Spaniard Dani Moreno on the final climb to ease to victory.
Behind him a tactical battle began amongst the race favourites as Contador attacked in a bid for the leader's red jersey.
Quintana and Joaquim Rodriguez reacted in time to catch the two-time Vuelta winner, but Froome and Valverde were left behind.
"The team worked very hard today and my legs felt good. The conditions favoured us to allow me to become the leader," said Quintana.
"I didn't do a lot. I finished along with the other favourites and we continue almost like it is 0-0.
"The idea is to stay in red all the way to Santiago, but if not then hopefully I will be after the last day.
"The objective is this year to make the podium at least to complete the podium at all three grand tours at 24 years old and go for the Tour de France another year."
There is a brief respite for all the riders in the first rest day of the race on Monday before the action gets back underway with a 36.7km individual time trial from Real Monasterio de Santa Maria de Veruela to Borja on Tuesday.
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