The NFL's Dallas Cowboys, up three spots from last year, and Major League Baseball's New York Yankees, up from fourth place in 2014, were tied for second with a value of $3.20 billion.
Global soccer powers Barcelona ($3.16 billion) and Manchester United ($3.10) rounded out the top five.
The NFL led all sports with 20 franchises in the top-50, followed by MLB (12), the National Basketball Association (10) and soccer (7).
Formula One's Ferrari (1.35 billion) were ranked 32nd while the Toronto Maple Leafs ($1.3 billion), tied in 37th, were the only National Hockey League team on the list.
According to Forbes, which began valuing sports franchises in 1998, the world's 50 most valuable teams are worth an average $1.75 billion, up 31 percent from 2014.
Forbes also said the minimum valuation to make the cut rose to $1.15 billion, up from $856 million a year ago, due largely to the soaring worth of NBA and MLB teams.
The 10 most valuable teams (in billions):
1. Real Madrid (Soccer) - $3.26
2. Dallas Cowboys (NFL) - $3.20
2. New York Yankees (Baseball) - $3.20
4. Barcelona (Soccer) - $3.16
5. Manchester United (Soccer) - $3.10
6. Los Angeles Lakers (NBA) - $2.60
6. New England Patriots (NFL) - $2.60
8. New York Knicks (NBA) - $2.50
9. Los Angeles Dodgers (Baseball) - $2.40
9. Washington Redskins (NFL) - $2.40
(Reporting by Frank Pingue in Toronto; editing by Toby Davis)
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