The Portugal captain limped out of the 1-1 draw at Real Valladolid on May 7 with an apparent thigh strain and missed Sunday's 2-0 defeat at Celta Vigo that ended Real's chances of winning La Liga.
With the Champions League final against Atletico Madrid on May 24 looming, his return is good news for the club as they chase a record-extending 10th continental crown.
"Cristiano Ronaldo is fine, available, and tomorrow he will play the match," Ancelotti told a news conference.
Only three players, goalkeeper Diego Lopez, centre back Pepe and long-term injury casualty Jese are unavailable for the game at the Bernabeu, the Italian added.
This season's championship will be decided at the Nou Camp on Saturday when second-placed Barcelona (86 points) host leaders Atletico (89).
A point would be enough for Atletico and Barca need to win to secure the title by virtue of a superior head-to-head record, used to separate teams if they are level on points.
(Reporting by Iain Rogers, editing by xxxxxxxxx)
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