"After some really excellent training this year, the start to the season has been riddled with a series of troublesome injuries, most recently a problem with an ankle ligament," Rutherford said in a statement on his verified Twitter account.
"As a result, I am absolutely gutted to say that I am unable to compete at the Anniversary Games this weekend -- one of the greatest events in our sport."
"Obviously this isn't ideal, but I am doing everything to give myself the best chance of success at the World Championships in London... "
The former Olympic champion is scheduled to defend his title at the World Championships being held from Aug. 4-13.
(Reporting by Aditi Prakash in Bengaluru)
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