Belgium's Gilbert finished a narrow second to the Omega Pharma Quick Step rider at the finish at Mairena del Aljarafe, with Germany's Robert Wagner leading the bunch a second later in third.
After three bunch stages, on Saturday the race returns to hillier terrain, with a summit finish at Altas de Penas Blancas. The race finishes on September 15 in Madrid.
(Reporting by Alasdair Fotheringham, editing by Pritha Sarkar)
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