The city's security committee for sports events proposed the ban "due to the heated rivalry between the two clubs, which already developed in the past into seriously critical situations for public order".
The away section of the stadium was closed when the two sides met in Turin and Naples last season for the same reason.
The fixture will be Juventus striker Gonzalo Higuain's first match against his former club since he completed an acrimonious 90 million euro (£80.03 million) transfer before the start of season.
The move infuriated a section of Napoli's fans, who tore up pictures of the 28-year-old and set fire to club shirts with his name on.
Table toppers Juventus travel to third-placed AC Milan on Saturday while Napoli, fifth in the standings, face bottom club Crotone on Sunday.
(Reporting by Silvia Recchimuzzi in Gdynia; Editing by Toby Davis)
