Combatants will be required to say whether they will agree to a so-called "cessation of hostilities" in the five-year war, with a proposed halt to fighting from tomorrow morning, AEDT.
Aid groups are hopeful it could be the lifeline desperately needed to get supplies through to Syria's civilian community.
The United Nations' Syria envoy, Staffan de Mistura, abruptly aborted a first round of talks earlier this month.
He urged countries in the International Syria Support Group, led by the United States and Russia, to do more preparatory work.
But the meeting had pushed for a ceasefire, resulting in a deal on a "cessation of hostilities" President Bashar al-Assad's government and the opposition both said they could support.
The co-chairman of the UN Taskforce on Humanitarian Access in Syria says a planned break in fighting this weekend should rescue civilians from what he calls "the abyss".
Jan Egeland says major and regional powers must use their influence on the warring parties to win clearance for UN aid convoys to reach besieged towns.
Mr Egeland says, after five years of war, the civilian population of Syria cannot take any more.
"You learn to be a realist, having worked as I have now with Syria since the beginning of the conflict. But I would say we're very hopeful -- very, very hopeful -- that things will now be better. There will be a multitude of problems still, but we've made more progress in these last weeks, at least in access, than in a long time. Next week should, with this cessation of hostilities, make us go to new strengths. And I'm repeating, the civilian population of Syria, they cannot sustain more."
Continued unrest has placed enormous pressure on aid deliveries.
Mr Egeland says the UN World Food Program's airdrop of food this week in the eastern town of Deir al-Zor ran into problems.
He says four of the 21 pallets with provisions were damaged, 10 went unaccounted for and seven landed in no-man's-land.
"There were two problems. Pallets were drifting in with their parachutes, so that some missed targets. And others of the pallets, the parachutes did not open, and the food was destroyed. So, with those lessons, the best humanitarian logisticians in the world are working on how to make this lifeline to 200,000 people in Deir al-Zor, mostly women and children, work. And the World Food Program is planning a three-month program."
The UN Special Envoy for Syria, Staffan de Mistura, says the ceasefire is vital to help desperate civilians receive crucial supplies.
"More needs to be done, more effectively, and, certainly, if, as we all hope, the cessation of hostilities takes hold effectively, that will have an impact on the acceleration of reaching the people in need in Syria, not only in the besieged areas but everywhere."
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