"Jack Wilshere won't feature against Italy and has now withdrawn from the group," the FA said on Twitter.
Liverpool defender Joe Gomez, who was forced off in the opening 10 minutes against Netherlands with an ankle injury, will remain with the England squad for further assessment.
England open their World Cup Group G campaign in Russia against Tunisia on June 18 before facing Panama and Belgium.
(Reporting by Aditi Prakash in Bengaluru, editing by Ed Osmond)
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