Wimbledon is the only grand slam tournament where the 23-year-old Dane has failed to reach the last eight, a wait which will stretch into another year after she went down 6-2 7-5 to the unseeded Czech in Monday's fourth round.
Missing the opportunity to make a grasscourt breakthrough was not lost on Wozniacki even though second seed Li Na had also been sent packing by Strycova in the previous round.
"There was no rhythm out there. I didn't serve as well as I have earlier in the tournament. That kind of makes it harder on grass," the 16th seed told reporters.
"Even when I had some opportunities I made some stupid mistakes or didn't play the balls properly. It was kind of one of those matches ... I guess sometimes that happens.
"You just have to get yourself back up and keep working and come back next time."
After a quick surrender of the opening set, Wozniacki dug deeper in the second, saving five match points before world number 43 Strycova booked a place in her first grand slam quarter-final.
The 28-year-old, who also toppled number 32 seed Elena Vesnina of Russia in the second round, next meets fellow Czech Petra Kvitova, the sixth seed, or China's Peng Shuai.
(Editing by Martyn Herman and Josh Reich)
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