Belgian defender Nicolas Lombaerts is fit to play against the Spaniards but experienced right back Alexander Anyukov remains doubtful.
"Lombaerts trained well yesterday but we'll need to carefully assess the condition of Anyukov as he was unable to take part in the last two training sessions," Spalletti said.
Zenit are second in the group with five points from four matches, seven behind Atletico who have already sealed a place in the knockout stage. Porto are third with four and Austria Vienna have one.
(Writing by Angel Krasimirov; Editing by Justin Palmer)
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