Barack Obama joked he was "born in Kenya' on stage at his foundation's first conference, in reference to "birther" conspiracy theories that he was ineligible to become the 44th president of the United States.
The former president was hosting the Obama Foundation's first global summit in Chicago when he made the joke, which appeared to be a swipe at current President Donald Trump who was a high profile promoter of the "birther" conspiracy theory.
“The reason I’m so excited to see you all here today in part is because this is where I started,” he told the audience.
“This isn’t where I was born, I was born in Kenya,” Mr Obama said before quickly adding, “That’s a joke.”
His humour was met with a round of laughter from the crowd.
Obama was born in Hawaii in 1961, releasing a copy of his birth certificate during his time in the White House.
Donald Trump pushed the "birther" conspiracy for years, before admitting Obama was born in the United States.
Former President Barack Obama, right, and former first lady Michelle Obama arrive for the first session of the Obama Foundation Summit Tuesday, Oct. 31, 2017. Source: AAP
During the summit speech, Mr Obama also revealed he no longer encourages selfies because of social etiquette.
"For Michelle and myself, no selfies," he said.
"This seems trivial, but it's not. One of the weird things about being president is I found people were no longer looking me in the eye."
The two-day summit is Obama's largest since he left the White House in January and will include guest speaker Prince Harry of Wales.
The Obama Foundation aims to promote strong and engaging communities for youth.
