Agriculture Minister David Littleproud has doused claims the world's most popular weedkiller is unsafe, calling for calm after health concerns sprouted.
The Cancer Council wants an independent review into glyphosate, the active ingredient in Roundup, after it was linked to non-Hodgkin's lymphoma.
But Mr Littleproud said the Australian Pesticides and Veterinary Medicines Authority had determined the chemical was safe.
"The science of the independent regulator says this chemical is safe if you follow the instructions," the minister told Sky News on Tuesday.
"I just say to everybody use some common sense, follow the instructions and you'll be OK."
Debate over glyphosate was reignited in August after a Californian jury ordered agribusiness giant Monsanto pay $US289 million ($A399 million) in damages to a former groundskeeper dying of cancer.
Mr Littleproud said that case and others aired by the ABC's Four Corners on Monday highlighted excessive exposure.
"Home gardeners shouldn't get too worried about this, You're not going to get exposed to levels so long as you follow the instructions," he said.
The minister said farmers were using the chemical in a sensible way, adding agriculture had come a long way in how pesticides were used.
"I just say to everyone calm down ... and have faith that we have the best science in the world," Mr Littleproud said.
APVMA's review came after a 2015 report by the International Agency for Research on Cancer, a World Health Organisation body, found glyphosate was "probably carcinogenic to humans".
Labor seized on the concerns, demanding a Senate inquiry into the independence and decision-making of the pesticides agency.
"This issue is too important to the agricultural community, to Australia's farmers, and to consumers to be left unresolved," opposition agriculture spokesman Joel Fitzgibbon said on Tuesday.
Labor also wants to investigate the impact of moving the regulator from Canberra to Armidale in northern NSW.
"There is no doubt the government's decision to relocate the APVMA has impacted on its operations," Mr Fitzgibbon said.
But Mr Littleproud accused the opposition of playing politics, saying the agency's most recent assessment was conducted before the relocation started.
National Farmers' Federation president Fiona Simson said the scientific evidence overwhelmingly proved the chemical was safe.
"There is simply no alternative that is as safe and as effective as glyphosate, for these purposes," Ms Simson said.
